The cDNA sequence of human NAMPT was amplified by PCR from a pGex-6p-3-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, University of Missouri). The PCR products were being digested and cloned into a pET21a+ vector utilizing NdeI and XhoI restriction enzymes. Some extent mutation was introduced by the fast alter https://eduardoejnsw.myparisblog.com/27001018/5-essential-elements-for-arv-471