5 mL binding buffer and purified manually with 25 mL settled beads. Respective suppliers�?protocols were followed for his or her buffer compositions and volumes. Pierce Ni-NTA Magnetic Agarose had the best yield in comparison with beads from the other suppliers. Fig. one: Workflow of an ordinary protein purification treatment using https://www.quora.com/What-are-nucleic-acids-extraction/answer/Even-Sa?prompt_topic_bio=1