POD reacting with hydrogen peroxide (H2O2) catalyzes oxidative condensation of tyramide to create remarkably reactive intermediates, tyramide radicals. The activated tyramide covalently binds to electron-loaded moieties such as tyrosine, phenylalanine and tryptophan at or near the POD. The quick 50 %-life of the tyramide radicals restricts the deposition of tyramide3. https://eed22677654.blogolize.com/the-single-best-strategy-to-use-for-biotinyl-tyramide-69168841